The combinations of Hyphantria cunea NPV (HcNPV) and three different fluorescent brighteners BA,VBL, CBS-X were added to the artificial diet and the influence of synergists in concentration 25 ℃ and 14L /10D( h). The results showed that the fluorescent brightenersBA and VBL had higher insecticidal activity, CBS-X had notsignificant synergistic effect.

Hyphantria cuea nucleopolyhedrovirus (HcNPV) and several other nucleopolyhedrosis was extracted bydifferential centrifugation, and purified with sucrose gradient centrifugation method. Polyhedrin was gotten andanalysed by SDS - PAGE. It indicated thatmost of those p roteins had 32 KD p rotein band, and with 64 KD p rotein,and might be the main p rotein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virusp rotein in 18 KD department had a specific band.